F l y i n g

 L i g h t - S c a t t e r i n g

I n d i c a t r i x  

The Flying Light Scattering Indicatrix (FLSI) is an angular dependency of the light scattered from individual moving particles. Originally, the FLSI is measured with the Scanning Flow Cytometer (SFC). The optical scheme of the SFC allows measurement of the FLSI of individual particles at polar angles from 10° to 120° with integration at azimuthal angles from 0° to 360° and with angular resolution of better than 0.5°. To satisfy the rate of flow cytometric analysis (up to 1000 particles per sec), the FLSI method has been developed for absolute real-time determination of individual spherical particle parameters: size and refractive index. These parameters are calculated from the approximating equations that relate the particle characteristics to the FLSI parameters: fringe pitch, inverse and forward visibilities.
It has been shown that indicatrix visibility depends basically on the phase-shift parameter (ro) whereas fringe pitch depends on the size parameter (alpha). An analysis of these dependencies allowed the determination of the region in the alpha & ro map where particle characteristics can be derived unambiguously from indicatrix parameters. The area in alpha & ro and size & refr. index maps has been clarified where the Flying Light-Scattering Indicatrix method is available.

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